To investigate the regional arterial stress-strain distributions referenced to the zero-stress state. Morphometry and stress-strain properties were studied in isolated segments of the thoracic aorta (T), abdominal aorta (A), left common carotid arteries (C), left femoral arteries (F) and the left pulmonary arteries (P) in 16 male Wistar rats. The distension test was performed as pressure-volume experiments. The dimensions of the outer wall were obtained from digitized images of the arterial segments at different pressures and at no-load and zero-stress states. The results showed that the morphometric data, such as inner and outer circumferential length, wall and lumen area, wall thickness, wall thickness-to-inner whins ratio and normalized outer diameter referenced to different pressures differed between the five arteries (p<0.01). The opening angle was largest in the pulmonary artery and smallest in thoracic aorta (p<0.01). The absolute value of both the inner and outer residual strain and the residual strain gradient were largest in the femoral artery and smallest in the thoracic aorta (p<0.01). In circumferential and longitudinal direction, the arterial wall was stiffest in the femoral artery and in the thoracic aorta respectively, and softest in the pulmonary artery. These results show that the morphometry and biomechanical properties distribution on these five different arterial segments was much different.