Objective:to seek suitable condition for calcining bovine bone and to provide a kind of good heterologous organic material for repairing bone defect. Methods: bovine bone slices (40mm×5mm×2.5mm) were calcined at various temperature and lasted for various period of time. Then the following properties of the bovine calcined bone slices were tested: porosity rate, hydrophilism, density, bending strength, mineral composition and heat analysis. Results: the major mineral composition of bovine calcined bone was HA of low crystallization. the suitable condition for calcining were the tempreture of 450℃ and lasted for about 32 hours, when the porosity rate of bovine calcined bone was 50.64% and its bending strength was 17.57Mpa. Otherwise, the physical property of bovine calcined bone would be decreased and some immunity would be remained. Conclusion: the suitablecalsined condition was at the temperature of 450℃ for about 32 hours. the major mineral composition of this heterologous organic bone was HA. it retained original frame of bovine bone and some mechanical strength. it has potential capability of repairing bone defect of conductivity.