The main types of bone tissue cells include osteoblasts, osteoclasts, and osteocytes, which work together with the extracellular matrix to maintain the structural integrity and functionality of the bone. Osteoclasts are specifically responsible for bone resorption, degrading the bone matrix through the release of acidic substances and proteolytic enzymes. Osteoclasts coordinate with osteoblasts to maintain bone homeostasis. An increased number of osteoclasts leads to an increased bone resorption, which can cause osteoporosis and other skeletal diseases; deficiency in osteoclast generation can lead to a weakened bone resorption and related diseases, such as osteopetrosis. Therefore, a precise regulation of osteoclast function plays a crucial role in maintaining the balance of bone homeostasis. Previous studies have mostly explained and elaborated on the regulation of osteoclasts by various biological factors from a bio-chemical perspective. However, more and more studies have confirmed that mechanical stimulation plays an important role in the differentiation process of osteoclasts. This review focuses on the impact of mechanical stimulation on osteoclast differentiation, discusses the possible roles it may play, and explores the new discoveries and future development in this field.