Abstract: Objective To investigate whether the Virtual Box Block Test (VBBT) we designed by a haptic device with virtual reality (VR) technology can be used for remote evaluation of upper limb motion function. Methods We designed a VBBT system for UE motor function assessment. This system can be used to calculated the VBBT score, mean velocity, spectral arc length, energy consumption and mean grasping force for the subjects. In total 20 healthy subjects and 16 post-stroke patients were recruited to perform the VBBT. Results Significant differences were found in all VBBT parameters (P ≤ 0.02) between post-stroke patients and healthy subjects. Receiver operating characteristic curve (ROC) analysis showed that the VBBT parameters were possible to distinguish the two groups (area under the curve (AUC) ≥ 0.709，P ≤ 0.033). The correlations between VBBT parameters and traditional clinical assessments indicated the concurrent validity of the VBBT. Additionally, the findings of multiple regression analysis suggested that the kinematic and kinetic parameters of the VBBT can partially explain the variance of the traditional clinical assessment. Conclusions The VBBT can be used as a remote assessment of upper limb motor function in post-stroke patients.