Objective Currently, the mainstream intraocular pressure(IOP) measurement devices in the market are contact and irritant, which can easily bring discomfort to patients and even lead to corneal infection during use. To solve this problem, an non-contact measurement method of intraocular pressure based on ultrasound imaging was proposed. Methods The method is verified by finite element analysis and experiment, and the simulation results of corneal deformation are given. A simulated eye was made and an experimental system for measuring IOP was built. A mathematical model of IOP, corneal deformation and pressure was established and its accuracy and repeatability were verified. Results The experiment results show that the flattened diameter and vertex displacement of the cornea have power and linear relationship with pressure under a certain IOP; both flattened diameter and vertex displacement have power relationship with IOP under constant pressure, which can be used as the index of IOP measurement. The verification results indicate that the average relative error of IOP measured by this method is less than 8%, and the relative average deviation is less than 9%. Conclusions The measurement results of the method are accurate and have good repeatability. The results of this study provide an effective way for non-contact measurement of IOP, which has a good application prospect.