Periodontium is mechanoresponsive to multiple types of mechanical stimuli like occlusal and orthodontic force and reacts quickly. It is widely used as the loading subject in researches regarding dental mechanical force models both in vivo and in vitro. This review summarized various animal models and cell culture loading methods (including static gravity approach, centrifugation approach, vibration approach, cyclical tension approach, fluid flow approach)，as well as parameters for periodontium in recent years, so as to provide references for the study of periodontal mechanoresponsive mechanism and the development of new clinical therapies.