可控牵张应力对山羊腰椎间盘退变的影响
DOI:
作者:
作者单位:

1.南华大学附属第一医院;2.重庆郭昌毕中医骨伤科医院;3.苏州大学

作者简介:

通讯作者:

中图分类号:

基金项目:


Effects of Controllable Tensile Stress on Lumbar Disc Degeneration in Goats
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:探讨轴向牵张应力对山羊IDD的影响。方法:选取24只6-8月龄雄性山羊,采用弹性应力装置以40N压缩应力轴向诱导构建山羊IDD模型,在IDD模型构建完成后,随机分为6组(A-C组为对照组,D-F组为实验组),并将压缩应力转换为牵张应力,A-C组牵张应力0 N,D-F组牵张应力40 N,牵张时间分别为4周、6周、8周,利用影像学(X线、MRI)及病理组织学(HE染色、免疫组化)评估退变及再水化程度。结果:对照组:随着时间的延长DHI、Pfirrmann分级均无统计学意义(P>0.05),实验组:随时间延长DHI高度增加有统计学意义(P<0.05),Pfirrmann分级随时间延长分级由低向高变化(V→III→IV);HE染色椎间盘髓核体积增大、髓核细胞增多。免疫组织化髓核I型胶原染色由棕色→黄色→淡黄色转变,6周后又向黄色转变I型胶原蛋白增加;髓核II型胶原染色由无色或淡黄色→黄色转变,6周后又向淡黄色转变II型胶原蛋白减少。结论:一定牵张应力在合适时间内轴向诱导山羊IDD模型可以修复并再水化,但是轴向牵张时间过长又重现椎间盘退变。

    Abstract:

    Objective: To investigate the effects of controllable tensile stress on lumbar disc degeneration in goats. Methods:Twenty-four male goats aged 6-8 months were selected to construct the IDD model by axial induction of 40N compression stress with elastic stress device. After the construction of the IDD model, they were randomly divided into 6 groups (Group A-C was the control group, and Group D-F was the experimental group). The compression stress of the IDD model was converted into tensile stress, and the traction stress of Group A-C was 0 N. The tension stress of group D-F is 40N. In 3 time periods (4 weeks, 6 weeks, 8 weeks), X-ray and MRI were used to observe the disc height and degree of degeneration (DHI, Pfirrmann grading assessment), pathological observation was made of the changes of HE staining, Ⅰ, immunohistochemical staining of type I collagen in the intervertebral disc. Results: In the control group, DHI and Pfirrmann grading showed no statistical significance with time extension (P > 0.05), while in the experimental group, DHI height increased with time extension with statistical significance (P < 0.05). Pfirrmann grading changed from low to high with time extension (V→III→IV). HE staining increased the volume of nucleus pulposus and increased nucleus pulposus cells. Immunohistochemical staining of type I collagen in nucleus pulposus changed from brown to yellow to light yellow, and 6 weeks later, the type I collagen was increased. The type II collagen staining of nucleus pulposus changed from colorless or light yellow to yellow, and the type II collagen decreased to light yellow 6 weeks later. Conclusion: The axial induced IDD can be repaired and rehydrated in a certain amount of time under a certain tensile stress, but the change of IDD will occur again if the axial tensile time is too long.

    参考文献
    相似文献
    引证文献
引用本文
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2021-10-18
  • 最后修改日期:2021-12-21
  • 录用日期:2021-12-22
  • 在线发布日期:
  • 出版日期:
关闭