短期跳跃负荷下兔髌腱起止点微细结构变化及炎性因子表达特征
DOI:
作者:
作者单位:

1.苏州大学 体育学院;2.西安交通大学城市学院;3.北京体育大学 运动医学与康复学院

作者简介:

通讯作者:

中图分类号:

基金项目:


The Effect of Short-term Jumping Load on the Microstructure and Expression of Inflammatory Factors of Bone-patellar Tendon Junctions in Rabbits
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的 探讨短期跳跃负荷下,兔髌骨髌腱结合部(PPTJ)与胫骨髌腱结合部(TPTJ)的适应性反应。方法 19只18周龄雌性新西兰大白兔,随机分为对照组(CON,n=4)与跳跃1天(J1)组、3天(J3)组、5天(J5)组(每组n=5)。使用电刺激定量跳跃装置使兔每日完成150次跳跃(10组×15次,每组3min,第4组与第8组结束后休息6min,其余组结束后休息3min,平均跳跃速度0.083次/s,每日训练总时长约70min),训练结束24h后处死动物,取左右后肢膝关节相应组织,行常规H&E染色与免疫组化处理,定性描述组织形态学结果并定量监测细胞密度、软骨带厚度与IL-1β、IL-6、TGF-β1表达量。结果 跳跃组(J1、J3和J5组)的PPTJ与TPTJ均发生不同程度的微损伤;J5组PPTJ中IL-1β和IL-6表达量均显著高于CON组与J1组;J3组PPTJ中IL-6表达量显著高于CON组;J5组TPTJ软骨带厚度显著高于CON、J1、J3组;CON组PPTJ的IL-1β与IL-6表达显著少于TPTJ;J1组PPTJ的IL-1β、TGF-β1、IL-6表达量显著少于TPTJ;J3组,PPTJ的IL-6表达量显著少于TPTJ。结论 短期跳跃负荷致兔髌骨髌腱结合部病理改变早于胫骨髌腱结合部,在短期跳跃负荷下的受损过程中,兔髌骨髌腱结合部炎性因子IL-1β、IL-6上调,而兔胫骨髌腱结合部则无明显炎性因子变化。本研究为髌腱病的发病机理提供实验室依据。

    Abstract:

    Objective To investigate the adaptive response of patella patellar tendon junction (PPTJ) and tibial patellar tendon junction (TPTJ) in rabbits under short-term jumping load. Methods Nineteen 18 week old female New Zealand white rabbits were randomly divided into control group (CON, n = 4) and jump 1 day (J1), 3 days (J3), 5 days (J5) group (each group n = 5). Electrical stimulation quantitative jumping device was used to make rabbits complete 150 jumps per day (10 groups × 15 jumps, 3 minutes per group, 6 minutes rest after the 4th and 8th groups, 3 minutes rest after the other groups, the average jumping speed is 0.083 times/ s, the total training time per day is about 70 minutes), the animals were sacrificed 24 hours after the training. The corresponding tissues of the left and right hind limbs were taken for H&E staining and immunohistochemistry. The histomorphological results were qualitatively described, and the cell density, cartilage thickness, and the expression of IL-1β, IL-6, and TGF-β1 were quantitatively monitored. Results The micro-injury of PPTJ and TPTJ occurred in different degrees in the jump groups; the expressions of IL-1β and IL-6 in PPTJ of the J5 group were significantly higher than the CON and J1 groups; the expression of IL-6 in PPTJ of the J3 group was significantly higher than the CON group; the thickness of TPTJ cartilage band in the J5 group was significantly greater than the CON, J1, and J3 groups;in the CON group, the expressions of IL-1β and IL-6 in PPTJ were significantly lower than TPTJ; in the J1 group, the expressions of IL-1β, TGF-β1, IL-6 in PPTJ were significantly lower than TPTJ; in the J3 group, the expression of IL-6 in PPTJ was significantly lower than TPTJ. Conclusions The pathological changes of patella patellar tendon junction induced by the short-term jumping load are earlier than those of tibial patellar tendon junction. The inflammatory factors IL-1β and IL-6 are up-regulated in the process of patella patellar tendon junction injury under short-term jumping load, but there is no significant change of inflammatory factors in tibial patellar tendon junction.

    参考文献
    相似文献
    引证文献
引用本文
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2021-10-12
  • 最后修改日期:2021-12-17
  • 录用日期:2021-12-20
  • 在线发布日期:
  • 出版日期:
关闭