1.晋中学院 体育系;2.武警山西总队医院 外一一科
目的 探讨针刺联合五步复位法通过MicroRNA-27a对游泳运动员腰椎盘突出生物力学研究。方法 选取符合条件要求的80例腰椎盘突出患者,将其随机分为四组,空白对照组(Con组)、针刺组(ZC组)、五步复位推拿组(TN组)、针刺联合五步复位法组(ZT组),每组20人,各治疗两个疗程,共一个月。治疗完毕后评价针刺、五步复位推拿及针刺联合五步复位推拿治疗效果。观察指标包括下腰痛JOA量表、McGill疼痛量表、生活质量QOL量表、重力矩和肌肉矩。同时,取各组患者治疗前后外周静脉血,通过荧光定量PCR检测miR-27a信号通路相关基因表达水平。结果 治疗前,各组患者年龄、病程、性别等基本信息无明显差异(P>0.05),具有可比性；干预治疗后,与空白对照组相比,针刺组、五步复位推拿组、针刺联合五步复位推拿组患者JOA、McGill、QOL、重力矩和肌肉矩均有显著改善(P<0.05),且联合治疗组改善效果更为显著(P<0.01)；荧光定量PCR结果显示,干预治疗后,与空白对照组相比,针刺组、五步复位推拿组、针刺联合五步复位推拿组静脉血中miR-27a(P<0.05),且联合治疗组上调更为显著(P<0.01)。结论 针刺联合五步复位法通过MicroRNA-27a信号通路提高游泳运动员腰椎盘突出生物力学。
Objective To explore the biomechanical study of acupuncture combined with five-step reduction method on swimming athletes with lumbar disc herniation through MicroRNA-27a. Methods 80 patients with lumbar disc herniation meeting the requirements were selected and randomly divided into four groups, blank control group (Con group), acupuncture group (ZC group), five-step reduction massage group (TN group), and acupuncture combined five-step reduction method group (ZT group), 20 people in each group, two courses of treatment for a total of one month. After the treatment, the therapeutic effects of acupuncture, five-step reduction massage, acupuncture combined with five-step reduction massage were evaluated. Observation indicators include low back pain JOA scale, McGill pain scale, quality of life QOL scale, gravitational moment and muscle moment. At the same time, the peripheral venous blood of each group of patients was collected before and after treatment, and the expression levels of miR-27a signaling pathway-related genes were detected by fluorescence quantitative PCR. Results Before treatment, there was no significant difference in basic information such as age, course of disease, gender, etc. Among the groups of patients (P>0.05), which was comparable; after intervention treatment, compared with the blank control group, the JOA, McGill, QOL, gravitational moment and muscle moment of patients in the acupuncture group, five-step reduction massage group, acupunctu recombined five-step reduction method group were significantly improved (P<0.05), and the improvement effect in the combined treatment group was more significant (P<0.01); Fluorescence quantitative PCR results showed that after intervention treatment, compared with the blank control group, miR-27a in the venous blood of the acupuncture group, five-step reduction massage group, and acupuncture combined with five-step reduction massage group were significantly up-regulated (P<0.05), and the combination treatment group was up-regulated more significantly (P<0.01). Conclusion Acupuncture combined with five-step reduction can improve the biomechanics of swimmers" lumbar disc herniation through the MicroRNA-27a signal pathway.