保存时间对兔髌韧带压缩力学性能的影响
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1.大连理工大学 工程力学系生物与纳米力学实验室;2.大连市中心医院 运动医学科;3.大连市骨科医院;4.大连西岗王鹏口腔诊所

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Effects of preservation duration on compressive mechanical properties of rabbit patellar ligament
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    摘要:

    目的 研究保存时间对兔髌韧带压缩力学性能的影响,以期为韧带的离体保存提供参考信息。方法 通过万能拉伸试验机,测试兔髌韧带在-20°C下经不同保存时间(36 d内)后的压缩力学性能,通过扫描电镜(SEM)观察胶原纤维的微观形态,差示扫描量热法(DSC)检测胶原纤维的热焓和变性温度。结果 随着保存时间的增加,髌韧带在40%应变下的压应力由0.019 MPa增至0.112 MPa,随后降至0.057 MPa。SEM下观察发现胶原纤维交联先增强后减弱。DSC结果显示热焓由59.47 J/g增至67.10 J/g,随后降至54.43 J/g;而变性温度由67.62°C增至77.28°C,随后降至64.10°C。结论 兔髌韧带在-20°C下保存,随着保存时间的增加,韧带的抗压强度先增强后减弱。这可能是胶原纤维的交联程度差异所致,胶原纤维的交联越强,压缩力学性能越强。

    Abstract:

    Objective The effect of preservation duration on compressive mechanical properties of rabbit patellar was studied with the attempt to provide reference for ligament preservation. Methods The compressive mechanical properties of rabbit patellar ligament preserved at -20°C after different preservation duration (in 36 d) were tested with the universal tensile testing machine. The microscopic morphology of collagen fiber was observed on the scanning electron microscopy (SEM). The enthalpy and denaturation temperature of collagen fibers was measured with differential scanning calorimetry (DSC). Results With the increase of preservation duration, the compressive stress of the patellar ligament at 40% strain increases from 0.019 MPa to 0.112 MPa and then decreases to 0.057 MPa. SEM shows that the cross-linking of collagen fibers is initially strengthened and then weakened. DSC results show that the enthalpy increases from 59.47 J/g to 67.10 J/g and then decreases to 54.43 J/g.The denaturation temperature increases from 67.62°C to 77.28°C and then decreases to 64.10°C. Conclusions With the increase of preservation duration, the rabbit patellar ligament’s compressive stress at strain of 40% increases firstly and then decreases. According to DSC and SEM, the reason for this change may be the variation of cross-linking level of collagen fibers. The stronger the cross-linking of collagen fibers, the stronger the compressive mechanical properties

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  • 收稿日期:2021-05-17
  • 最后修改日期:2021-06-25
  • 录用日期:2021-06-30
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