目的 构建可用于单细胞培养的可折叠微板，建立微板折叠的有限元模型，给出单细胞三维收缩时牵张力的计算方法。方法 基于可折叠微板上单细胞培养过程中细胞铺展产生的牵张力引起微板发生折叠的角度，测量细胞牵张力引起微板折叠的角度，利用有限元模拟得到弯矩与折叠角之间的关系以及牵张力跟弯矩之间的关系，从而实现表征单细胞三维状态下的牵张力。结果 HSF和MC3T3-E1两种类型细胞在三维状态下的单细胞折叠角度范围分别在73°~173°和49°~138°，由此测算出的细胞牵张力大小范围分别在55~210 nN和52~161 nN。 结论 本文构建单细胞培养的可折叠微板表征单细胞在三维结构中牵张力大小的方法，对进一步发展三维状态下细胞粘附、迁移和铺展中牵张力的计算具有一定参考价值。
Objective To design and fabricate a foldable microplate for single cell culture, and establish the finite element model of the folding microplate to calculate the traction force of single cell when it contracted in three-dimensional state. Methods Based on the experimental data of single cell cultured on the foldable microplate, the folding angle of the microplate was calculated. The finite element simulation was used to calculate the bending moment of the microplate, and the function relation between bending moment and folding angle M=M(θ) was obtained. Then the relation between traction force and bending moment F= F (M) was derived. Results The folding angle of the microplate with HSF and MC3T3-E1 were determined among the range of 73°~173° and 49°~138°, respectively. The single-cell tractive forces of HSF and MC3T3-E1 were calculated in the range of 0-250 nN and 50-150 nN, respectively. Conclusions A new method for measuring the traction force of a single cell in a three-dimensional structure is proposed. This method provide a new insight for further development of the calculation of the traction force of cells in the three-dimensional physiological-related adhesion, spreading and migration behavior.