Abstract: the purpose of this study is to evaluate the difference of foot and ankle function of subjects with different foot types from both dynamic and static aspects by means of exercise biomechanics, so as to provide theoretical support and reference for the study of foot and ankle movement. Methods: sixty-five subjects were divided into three groups: flat foot, low foot arch and normal foot according to arch index. One-way ANOVA was used to analyze the difference of static and dynamic foot and ankle function indexes among the three groups. Results: in terms of static ankle function, there was significant difference in ankle adaptability between normal foot and low arch and flat foot (P<0.05), and between normal foot and flat foot in longitudinal arch adaptability (P<0.05). There was no significant difference in transverse arch adaptability among the three groups. In the dynamic function of foot and ankle, there were significant differences in the amplitude and time of force deviation between normal foot and low arch and flat foot in the buffer stage (P<0.05), and in the transition stage, compared with low arch and flat foot, the transition time of normal foot was significantly lower than that of low arch and flat foot (P<0.05). In the pedal-extension stage, there were significant differences in pedal-extension time and pedal-extension speed between normal foot and flat foot (P<0.05). Conclusion: in terms of static foot and ankle function, the posture maintenance ability and longitudinal arch shape maintenance ability of low arch and flat foot are worse than that of normal foot when load is increased, and in terms of dynamic ankle function, the arch rigidity and plantar elasticity of low arch and flat foot are worse than that of normal foot, and the normal foot shows better load-bearing cushioning ability, elastic-to-rigid transition ability and pedal extension efficiency when walking. A systematic and comprehensive evaluation system is of great significance to the prediction of foot and ankle injury risk and the evaluation of sports ability.