Objective: The purpose of this study was to investigate the biomechanics effects of shoes type and walking speed on the knee when walking at a different speed and wearing different shoes or barefoot, and to provide a theoretical reference for scientific fitness. Methods: Vicon nexus motion capture system (200 Hz) and Kistler force plate (1000 Hz) was used to collect lower limb kinematics and ground reaction force simultaneously from 10 subjects during walking at different speed and wearing different shoes. Visual3D was used for inverse dynamics calculation. Two-way (2 movements × 3 shoe conditions) repeated measures analysis of variance was used to statistically analyze each dependent variables. Results: Compared with jogging, the lateral excursion of plantar pressure center (COP) was greater (P < 0.05), the knee joint moment arm in frontal plane, the knee adduction moment and peak loading rate of knee joint were smaller (P < 0.05), but the angular impulse of knee joint in frontal plane was greater (P < 0.05); compared with ordinary running shoes, the lateral excursion of COP was greater (P < 0.05), and the knee joint moment arm in frontal knee joint, the knee adduction moment, the peak load rate and the knee adduction moment-time integral were smaller (P < 0.05). The results showed that the risk of knee injury was the least when barefoot jogging was performed in forefoot strike mode. In other hand, Minimalist shoes was recommended to the walkers who are not suitable for barefoot jogging.