Objective: We fused the ileum of porcine intestines with radiofrequency (RF) energy through a novel linkage-type pressure controlled electrode with the aim of verifying the feasibility and security of the RF energy tissue fusion technology. Methods: Fresh porcine intestines were fixed on the negative electrode in the order of "mucosa-serosa", and then different compressive pressures（497，796，995，1194，1492 kPa）and RF energy were applied to the tissue through positive electrode to complete anastomosis. Biomechanical properties of fused area were studied by tensile strength and bursting pressure test. At last, the thermal diffusion and tissue microstructure were studied to evaluate the effect of anastomosis. Results: The anastomotic tensile strength and bursting pressure can reach 8.73±1.11N and 62.2±3.08 mmHg respectively when the energy output power, pressure and welding time are 160 W, 995 kPa and 13s, respectively, and an intact microstructure with little free collagen in the fusion area can be observed. Conclusion: The technology of tissue fusion based on radiofrequency energy could accomplish fast and stable intestinal tract reconstruction, which shows great potential in clinical application. It is of great significance to shorten the operation time, simplify the operation process and improve the operation quality.