1.上海交通大学 海洋工程国家重点实验室;2.上海交通大学医学院附属仁济医院 胃肠外科;3.上海交通大学 生物医学工程学院
1.State Key Laboratory of Ocean Engineering,Shanghai Jiao Tong University;2.Department of Gastrointestinal Surgery,Renji Hospital,Shanghai Jiaotong University School of Medicine;3.School of Biomedical Engineering,Shanghai Jiao Tong University
目的 循环肿瘤细胞（circulating tumor cell，CTC）数目与病人预后及生存率密切相关，研究CTC在血液中的运动过程以及CellCollector取样针对CTC的捕获机理，揭示CTC检出数与体内实际浓度的关系。方法 基于Fluent和EDEM软件，应用单向流固耦合的两相流计算方法，建立了血流动力学模型及CTC组分运输模型，开展了若干CTC浓度工况下的捕获特性仿真。结果 CellCollector取样针的CTC捕获数与体内CTC的浓度呈明显的正相关。CTC浓度较低时，只能在少数时间区间内捕获到CTC颗粒，捕获有一定的偶然性；随着浓度增加，CTC捕获数在时间上的均匀性变好，同时捕获总数也随之增加。结论 通过对仿真结果的拟合，初步给出了CTC捕获数与体内浓度的量化解析关系，为CellCollector的临床使用提供了理论依据及力学机理阐释。
Objective The number of circulating tumor cells (CTCs) is closely related to the prognosis and survival rate of patients. To study the movement process of CTCs in the blood and the mechanism of CTC capture by CellCollector, and to reveal the relationship between the number of detected CTCs detected and the actual CTC concentration in the body. Methods Based on Fluent and EDEM software, unidirectional fluid-solid interaction method was applied to establish a two-phase flow model, including the hemodynamic model and the CTC transport model, and the capture simulations under several CTC concentration conditions was were carried out. Results The number of CTCs captured by CellCollector is significantly positively correlated with the CTC concentration in the body. When the CTC concentration is low, CTCs can only be captured in a few time intervals, and the capture has a certain contingency; as the concentration increases, the uniformity of CTC captures over time becomes better, and the total number of captures also increases. Conclusion Through the fitting of the simulation results, the analytical quantitative relationship between the number of captured CTCs captured and the CTC concentration in the body is given, which provides a theoretical basis and mechanics explanation for the clinical use of CellCollector.