Objective: To study the effects of excitation source, intraocular pressure, and material parameters on the frequency response of the human eye under acoustic excitation. Method: Based on the three-dimensional model of the whole eye, the finite element analysis was performed. According to the deformation and stress distribution of the human eye, the amplitude value at resonance, the effects of various parameters on frequency response of the human eye were quantitatively analyzed. Results: When the intraocular pressure and material parameters are constant and the position and size of the excitation source are changed, the acoustic excitation source is placed directly above the cornea, and an offset of 45 °, and when the excitation is large, respectively. The amplitude value at resonance of the human eye at 134 Hz is 35μm, 48μm and 133μm, respectively. When the position and size of the excitation source and material parameters are fixed and the intraocular pressure is changed, the first-order resonance frequency is almost unaffected by the intraocular pressure, and the resonance frequencies of other orders will shift slightly to the left as the intraocular pressure increases, and the offset frequency value is smaller. When the location and size of the excitation source, intraocular pressure and corneal elasticity remain certain, and the scleral elastic parameters are changed, the resonance frequency of the human eye tissue increases with the increase of the scleral elasticity. Conclusion: The position and size of the excitation source have no effect on the resonance frequency of the human eye, but they have a greater effect on the the amplitude value at resonance. The material parameters of the human eye tissue have a greater effect on the frequency response, but the intraocular pressure has almost no effect.