Objective To investigate the effect of ankle joint taping (function of restriction: ankle varus and plantar flexion) on the knee biomechanical characteristics during the drop landing on the dominant foot, and to explore the effect of ankle joint taping on non-contact anterior cruciate ligament injury. Materials and Methods Eighteen recreational athletes completed this study. Subjects performed a dominant-legged drop landing under two different ankle conditions( control and taping). Vicon three dimensional motion capture system and one Kistler plate measured kinetic and kinematic data; Noraxon skin Surface Electromyography (EMG) system was recorded from six right dominant side muscles. Pair t-test analysis of was performed before and after taping. Results Ankle taping significantly increased flexion angle at initial contact and maximum flexion angle when compared to control; but ankle taping significantly decreased maximum valgus angle. Conclusion The way of restriction varus and plantar flexion can reduce the risk of anterior cruciate ligament injury. Ankle taping provided protective benefits for knee joint during drop landing.