Objective To develop a batch process method for kinematics data of mass population based on MATLAB. Methods Based on MATLAB, the original coordinate data of markers from motion capture system were first batch-read with interpolation processing for eliminating the error points. The body-fixed base of contiguous rigid body attached on human body was then constructed as the reference base and body-fixed base of relative joint kinematics computing. The flexion/extension, abduction/adduction, internal and external rotation angular displacement, angular velocity and angular acceleration of joints were obtained based on the rigid body kinematics, the basic description of rigid body posture and the direction cosine matrix. Results Taking climbing upstairs and squatting as samples, the joint kinematics of lower extremity were analyzed and calculated by the batch process method for kinematics data of mass population based on MATLAB to prove the effectiveness and accuracy of the method. Conclusions The batch process method for kinematics data of mass population based on MATLAB was proved to be effective and accurate, and can be provided as a statistical analysis tool for anthropometry, human engineering, etc.