Objective To report a self-developed cell carrier made of silicone rubber membrane, and to calculate the strain distributions on the membrane and analyze biocompatibility of the silicone rubber by three dimensional (3D) finite element method (FEM) so as to make a comprehensive evaluation on this silicon rubber membrane and provide theoretical basis for cellular research under stress stimulation. Methods The silicon rubber was made into a membrane with the thickness of 0.1 cm. Based on elastic modulus and Poisson’s ratio of the silicone rubber membrane, 3D FEM was used to simulate the deformation of the membrane induced by the stretch stress. MTT method was used to compare the growing situation of cells cultured on the silicone membranes and in standard plastic plates, respectively. Embedding experiment was also conducted to find out whether the material had biological toxicity or not. ResultsUnder the loading of 0.5%~20% strain, the area with effective strain was in the center of the membrane, accounting for 90% of the total area. There still existed some differences in biocompatibility between the silicon rubber membrane and standard plastic plate, but the material itself showed no toxicity. Conclusions Stress distributions on the surface of this self-developed silicon rubber membrane and its biocompatibility were generally acceptable for cell culture, but its surface should be further improved to fit for cell stress experiment.