Multi-component synthetical nanofiber is a result from blending nanotechnology, material science and biological science. The nanofiber represents a kind of classic nanostructures that can fully simulate the microenvironment of fibrous structure in vivo, while providing topographical structures, biomechanics and biochemistry agents to promote tissue regeneration and wound healing. These fibers are prepared with mixtures of naturally and synthetically derived polymers. They have synergistic effect on mechanical properties, biological response and structural properties and play an important role in tissue engineering and drug delivery.