Objective To study the deformation and stress distribution of femur after total hip arthroplasty (THA) and its influence on the vibration mode and natural frequency of femur. Method Two finite element models of natural femur and femur after THA were developed on the basis of computed tomography (CT) scans from a normal young man to investigate the biomechanical behavior of the subjectunder gait condition and make the modal analysis. Results (1) After THA, obvious stress concentration was obtained around the prosthesis neck, and the stress shielding was observed; (2) The peak stress of femur model after THA increased to 4.36 times of the original one; (3) The natural frequency for constrained mode was much higher than that of free mode; (4) With the increase of vibration mode, the differences in natural frequency between two models became larger; (5) Bending and twisting were the main vibration mode of femur, and there were no significant changes in vibration mode before and after THA. Conclusions The prosthesis could change the mechanical and structural properties of the original femur. In order to avoid prosthesis loosening derived from sympathetic vibration, the vibration property of femur must be taken into consideration in the design of prosthesis.