目的 采用机械拉伸方法并通过裱衬纤连蛋白（胞外基质成份）调节整合素受配体结合，探索整合素再分布变化对肝癌细胞黏附运动行为的影响，以及骨架重排对整合素再分布变化的影响。方法 运用免疫荧光染色、激光共聚焦显微技术和定量形态学分析法，观察整合素分布变化和细胞骨架装配调整，及对细胞运动变形能力进行检测和定量分析。结果 (1)不同形态细胞的整合素表达和分布特征不同，铺展细胞β1整合素表达高于未铺展的圆细胞，分布峰值为基底面，而圆细胞游离面的整合素分布反较基底面多。(2）机械拉伸5 h后不同形态细胞β1整合素表达均升高，分布带增宽；卸载1 h整合素表达下调，出现弥散分布倾向，其中以圆细胞最为明显。(3）裱衬FN后β1整合素表达增加，不同形态细胞整合素分布均向基底转移，并使肝癌细胞净迁移距离减小。(4)机械拉伸5 h后60％的细胞长轴取向集中在70°～110°之间，细胞骨架倾向垂直于拉伸方向排列,卸载1 h细胞骨架明显解聚。结论 肝癌细胞整合素分布变化受胞内骨架调整和胞外配体数量的影响，单个圆细胞的整合素分布特征与其较强的转移浸润能力相关。

Objective To explore the influence of integrin redistribution on hepatoma cell alignment and migration and the influence of cytoskeleton reassembly on integrin redistribution by the method of mechanical loadingunloading and fibronection(FN) coating. Method By using immuneofluescence staining, cofocal laser scanning microscopy and quantitative morphological analysis, integrin distribution change and crtoskeleton assembly adjustment were observed and the deformation of cell movement was tested and analyzed quantitatively. Results (1) cells with different forms have different integrin expressions and distribution features. The β1 integrin expression for spreading cells was higher than that for round (nonspreading) cells. For spreading cells, the strongest staining was found towards the attachment surface. While for round (nonspreading) cells, the integrin staining on the free surface towards medium was stronger than that towards the attachment surface. (2) After 5 hours of mechanical stretch, the β1 integrin expression for both spreading and round cells increased, and distribution peaks towards the attachment surface broadened. At 1 hour after unloading, the β1 integrin expression decreased and the distribution of integrin staining showed the tendency of dispersion, especially for round cells. (3) After coating the substrates with FN, the β1 integrin expression increased. The integrin staining for either spreading or round cells was more towards the attachment surface to reduce the migration of hepatoma cells. 4) After 5 hours of mechanical stretch, 60% of cells showed their orientation of major axes distributed between 70°～110° towards the stretching direction, and the cytoskeleton aligned vertically to the stretching direction. Cytoskeletons were found significantly depolymerized at 1 hour of unloading. Conclusions The change of integrin distribution is affected by cytoskeleton aligned and the number of ligand. The distribution feature of the whole integrin expression on the surface of individual round cells is related to their stronger invasion and metastasis capability.

国家自然科学基金资助项（10472137），国家211计划资助项目(B0623)