目的 研究溶胶-凝胶生物活性玻璃/胶原组织工程支架的力学性能及降解性能，为胶原基复合支架的进一步应用，提供理论基础。方法 以纳米溶胶-凝胶生物活性玻璃为添加相，利用冷冻干燥法制备了溶胶-凝胶生物活性玻璃/胶原基复合多孔组织工程支架；通过调整生物活性玻璃添加量来影响溶液中胶原纤维的聚集状态，从而改变复合支架的显微形貌，进而得到了不同力学性能的复合支架。结果 (1)溶液中胶原纤维的聚集状态，制备出具有直径约为400-600nm的粗胶原纤维束支架材料，这种粗胶原纤维束对改善胶原基组织工程支架的力学强度和减慢其降解速度具有重要作用。(2)利用FTIR和Raman等技术综合分析研究了溶胶-凝胶生物活性玻璃对胶原蛋白的二级结构的影响，当复合材料中胶原含量小于20%时，胶原蛋白二级结构破坏严重。结论 当胶原与生物活性玻璃质量比为40：60时，所制备的复合支架具有最好的抗压性能和降解性能，为进一步应用提供了研究基础。

Objective To investigate the mechanical prosperity and degradation rate of the scaffolds by compounding collagen and the nano sol-gel derived bioactive glass were studied in this paper，and that would provide the theoretical basis for the further application of collagen based scaffold. Method The scaffold by compounding collagen and the nano sol-gel derived bioactive glass (58S) were prepared using the freeze-drying techniques with the bioactive glass as phase addition. To affect the aggregation state of the collagen fibers with adjusting the supplementation of bioactive glass, then the microstructures of the compound scaffold would be different. At last, the compound scaffolds with different mechanical properties were prepared. Results (1) As the aggregation state of the collagen fibers changed, the scaffold with the coarser collagen fibers with the diameters 400-600nm approximately is prepared. The coarser collagen fibers would play an important role in improving the mechanical property and slowing down the degradation rate of the collagen based scaffolds. (2) The interactions between bioactive glass and collagen are studied by FTIR and Raman technologies. When the quality of content of collagen in the compound scaffold is lower than 20%, the secondary structure of collagen is damaged severely. Conclusions The composite scaffold with the mass ratio of collagen to bioactive glass to 40:60 has the best performance in mechanical property and degradation, which would be helpful for further applications

国家自然科学基金（50672028）;国家自然科学基金重点项目（50830101, 50732003）;浙江省自然科学基金（Y407241）