Objective Partial Foot Amputation (PFA) is the most commonly employed amputation in clinic. Analysis of gait changes induced by three typical amputation levels of PFA (Metatarsophalangeal joints, transmetatarsal, Lisfrane joints) will contribute to design suitable aid devices, such as orthopedic shoes, partial foot prosthesis, and so on. Method Measure the walk speed, step length, step frequency, peak plantar pressure, impulse, contact time and contact area with Footscan plantar pressure measure system and OPTOTRAK motion analysis system, and then compare these parameters among three amputation levels. Results The gaits are obvious different among these three different levels of foot amputation: the shorter the residual foot, the shorter the step and the more obvious the biped loading time difference; peak force occurs mainly at the metatarsal and the heel area for the MTP, while occurs at the midfoot and heel area for the TMA and Lisfrane amputation. Conclusions It is maybe important to take into account of amputation level when aid device is designed for PFA patient to improve the gait.