Objective tribological performance of the new bio-therapeutic lubricants is studied according to different composition concentration, including hyaluronic acid, albumin and alendronic acid sodium. Methods the bio-therapeutic lubricants were orthogonal experimental designed. Factors as friction coefficient, wear spot diameter and viscosity were investigated via a four-ball tribo-tester, viscosity meter and optical microscope. Results (1) hyaluronic acid has the most significant impact on the lubricant’s performance; (2) high albumin concentration will reduce friction, while increasing wear rate due to the possible tribocorrosion; (3) better lubricating performance is observed with higher alendronic acid sodium content. Conclusion bio-therapeutic lubricants partially recover the functions of synovial fluids and provide good lubricating property.