Objective To observe the stability of liquid resistance of cultured human ciliary muscle cells. Methods To observe the time for 7mm high liquid to penetrate through 3 groups of 8、14、20 days cultured 4 passage human ciliary muscle cells on Transwell (5 in each group). Results In groups of 14、20 days cells, the differences of penetrating velocity are within 15% among 60% Transwell、within 30% among 80% Transwell. Conclusion The resistance of cultured human ciliary muscle cells to liquid is almost stable.