Objective To establish a three-dimensional finite element analysis model of the maxillary first molar andvalidate the availability of the model in order to provide the biomechanics basis for prosthesis. Methods With the datumfrom Chinese digital human male No.1, a 3-D structures of enamel, dentin and elements pulp were constructed by thecombination of software Mimics9.1 and Freeform. The meshed models were acquired and finished with Ansys 8.0. Thestresses of the model were analyzed during the perpendicularity loading and the oblique loading, respectively. ResultThe model consisted of enamel, dentin, elements pulp, simulated periodontal membrane and simulated alveolar bone. Themesh division and the elements for each part of the model were: 8303 nodes and 5092 elements for ename; 104883 nodesand 77357 elements for dentin; 19818 nodes and 12116 elements for elements pulp; 2321 nodes and 2278 elements forperiodontal membrane; 31869 nodes and 25264 elements for alveolar bone. The effect of the perpendicularity loading andthe oblique loading indicated that the maximum force was at the 1/3 neck of root. Conclusions Combined usage ofMimics and Freeform, the finite element model constructed has the advantages of intact structure and precise elementswhich can simulate the natural condition and facilitate the further biomechanical research.