Objective To investigate biomechanics of Achilles tendon on an in vivo model. Methods 45 rabbits were averagely assigned into 3 groups at random. Group A (normal left Achilles tendon), Group B (normal right Achilles tendon), Group C (non-innervated left Achilles tendon). Their biomechanical variables were measured by modified buckle transducer. Result The tension-time curve can be mainly classified into four types (I, II, III, IV). Biomechanical variables of Achilles tendon under non-innervated condition were much lower than those under normal condition (P<0.05). Conclusion Modified buckle transducer can be used for measuring tension of Achilles tendon in vivo and be characterized as feasible, stable and sensitive.