Objective This experimental study aimed to evaluate biomechanical properties of human cadeavaric anterior longitudinal ligament of cervical spine for potential clinical use. Methods Anterior longitudinal ligament of C1-C4?C4-T1 segments was obtained from 8 fresh Chinese cadavers to test their tensile strength using Shimadzu AUTOGRAPH electronic universal testing machine. Results Mechanical properties of anterior longitudinal ligament of C1-C4?C4-T1 segments were obtained, including maximal tensile load and strain , Lagrange tensile stress and strain, Euler stress and elastic modulus With the least square method the stress and strain of the anterior longitudinal ligament of C1-C4?C4-T1 segments were plotted by stress-strain curve. Conclusion The stress of the C1-C4 segment was greater than that of the C4-T1 segment. There were no significant difference found in strain of the anterior longitudinal ligament of different segments. The modulus of elastic of the C4-T1 segment was however greater than that of the C1-C4 segment.