Objective A testing apparatus for shear stress of endothelial cells was developed ,which provided a experimental platform for studying the response of the biological characteristics of cultured endothelial cells to hemodynamic environment. Methods Applying the theories of the hydrodynamics and hemodynamics, a testing apparatus for shear stress to endothelial cells was developed. Results Under both normal and abnormal physiological conditions, control and adjust this apparatus can accurately the values of the shear stress, vertical stress and pulsatile amplitude ,pulsatile frequency of the vertical stress. Conclusion This apparatus can simulate hemodynamic environment coincided with the physiological condition of the body.