Objective The foot motion angles in 3D space were measured in this study. Methods Thermoplastic material were molded as the bracket of 3D marker triad, which can closely follow the contours of foot. This study used ALBA VISION ACAM50 3D motion analysis system to detect the marker position and calculate the foot motion angles in 3D space. Results The 3D foot motion pattern were properly described. Conclusion This method can be applied for research and clinical study of 3D foot motion.