Objective In order to solve the problem that only one kind of shear stress values could be observed during each parallel-plate flow chamber (PPFC) experiment, a kind of new PPFC - Two-Dimensional Bifurcation Parallel-Plate Flow Chamber (TDBPPFC) is proposed. Methods The distributions of the velocities inside and the shear stresses at the bottom of the chamber are given by computational simulation with laminar flow in the TDBPPFC. Results The results show that the mechanical behaviors of cultured cells undergoing the shear stresses with various values may be simultaneously observed by using this kind of new flow chamber. Conclusion These results possessed practical importance in optimum design and rational use of the TDBPPFC. And it was also helpful in analyzing the mechanical behavior of the cells and in discussing the effects of the shear stress distribution on the cells in the TDBPPFC.