Pullout test has been widely utilized in evaluating design parameters of screws. This is due to its simplicity in the experimental procedure.However, simple test creates some difficult problems. For example, no discussion has been made in the following topics:(1) effect of insertion position and direction,(2) effect of pullout direction, (3) effect of variation of cancellous bone structures. Insertion position and direction are relative to the geometric orientation of cancellous structures. Pullout direction can also contribute to pullout strength. Thinking differently, in this study, we use two kinds of trabecular structures: isotropic rod-type from bovine femoral condyle and plate-rod type structure from bovine spine vertebral body C_2～C_6, to check how this structural variation affecting the maximum pullout force in the pullout test. Finally, finite element modeling for pullout test is important based on several points: (1) how accurate can be obtained. (2) local deformation pattern around threads, (3) convergence characteristics. In the finite element analysis the strain energy density criterion will be used to define failure condition in the pullout simulation.